Literature Review: Analysis of fatty alcohol derivatives with comprehensive two-dimensional liquid chromatography coupled with mass spectrometry

Elsner, V.; Laun, S.; Melchior, D.; Köhler, M.; Schmitz, O. J. Journal of Chromatography A. 2012, 1268, 22–28.

This article describes the use of online LC x LC coupled with mass spectrometric detection for the analysis of anionic, non-ionic, and amphoteric surfactants with with different end groups in a single analysis. A HILIC separation is used in the first dimension followed by a reversed-phases separation in the second dimension. With this combination of phases, separation in the first dimension is based primarily on the degree of ethoxylation, whereas separation in the second dimension is based primarily on surfactant chain length. Modern HPLC components are used and the performance of two different systems composed of different components is compared. The interface between the two separation dimensions is a standard 10-port, 2-position valve.

Figures 7 and 8 are beautiful examples of separations with a high degree of orthogonality, and good separation performance in both dimensions. Unfortunately, no estimates of peak capacities of the separations are mentioned. Nevertheless, over 100 surfactant species are identified in separations of standard mixtures. The authors suggest that next steps should involve the analysis of these surfactants in commercial formulations such as cleaning agents.

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