Literature Review: Direct bioanalytical sample injection with 2D LC-MS

Cassiano, N.; Barreiro, J.; Oliveira, R.; Cass, Q. Bioanalysis. 2012, 4, 2737–2756. doi: 10.4155/bio.12.226

This recent review article with 178 references is an application oriented review of papers appearing in the time period 2008-2012 describing the use of various forms of two-dimensional liquid chromatography for bioanalysis, with a focus on those applications involving mass spectrometric detection. Tables 1 and 2 primarily describe applications of heartcutting or coupled column configurations (LC-LC) involving the use of Restricted Access Media (RAM) columns, and/or Turbulent Flow Chromatography (TFC) for sample cleanup in the first column. Table 3 summarizes proteomic applications involving comprehensive 2DLC (LC x LC). The article concludes with a discussion of the capabilities of different types of mass analyzers in the context of 2DLC separations.

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